Halophytes, the naturally salt-tolerant plants growing in high salinity soil, have a long history of usage for alimentation, for their medicinal value, for their high salt contents. Halophytes are receiving great attention as a valuable source to sequester carbon and to reduce salinity of soil and also as a cheap and promising source of bioactive secondary metabolites. In the course of searching for new substances that promotes hair growth and prevents hair loss from native halophytes, it was found that the methanolic extract of Artemisia fukudo Makino (Asteraceae) showed the potent activities. In human follicle dermal papilla cell (HFDPC), keratinocyte (HaCaT) and human umbilical vein endothelial cells (HUVEC) cells, the total methanolic extract of A. fukudo aerial parts significantly increased the expressions of vascular endothelial growth factor (VEGF), β-catenin, insulin like growth factor -1 (IGF-1) and p-glycogen synthase kinase (p-GSK) proteins compared to minoxidil, positive control. Eight compounds (1~8) were isolated from the bioactive fraction of A. fukudo extract, and the structures of isolated compounds were determined by 1D-, 2D-NMR and MS analysis. Compounds 1~8 were identified as compound 1 (new), compound 2 (new), reynosin (3), baynol C (4), scopoletin (5), jaceosidin (6), eupatilin (7) and jaceidin (8). Compound 3 and 4 are newly reported in nature and all compounds are reported for the first time from this plant. Among the comounds The isolated compounds 1~8 were evaluated for their activities in HFDPC, HaCaT and HUVEC cells. Among the compounds tested, reynosin (3), baynol C (4), scopoletin (5) and eupatilin (7) showed the most potent improvement in the expressions of β-catenin, IGF-1 and p-GSK in HFDPC, HaCaT and HUVEC cells. A. fukudo extract and its bioactive compounds are expected to have potential candidates that promote hair growth and prevents or treats hair loss.

Primary Presenter: Eun Ju Jeong, Gyeongsang National University (jeong.ej@gnu.ac.kr)

Authors:

Yun Na Kim, Gyeongsang National University  ()

Yu Jung Kim, Gyeongsang National Universit  ()

Min Gyu Park, Gyeongsang National Universit  ()

Jae Sun Lee, Gyeongsang National Universit  ()

Sun Woo Park, Gyeongsang National Universit  ()

Jung-Rae Rho, Kunsan National University  ()